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Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and <t>DAPI-stained</t> nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and <t>DAPI-stained</t> nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

doi: 10.1016/j.mtbio.2026.102808

Figure Lengend Snippet: Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Synthesis conditions were screened by staining LDs with BODIPY 493/503 (5 μM, MedChemExpress, USA) and counterstaining nuclei with DAPI (7 μM, Beyotime, China).

Techniques: Immunofluorescence, Fluorescence, Staining, Western Blot, Biomarker Discovery, Expressing